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Project Title: Invasion of Gemmamyces piceae in the Czech Republic. Distribution and importance of the pathogen and epidemiology of bud blight disease (LD15148)
Key words: Gemmamyces piceae – bud blight – distribution – epidemiology – systematics – control

State funding provider: Ministry of Education, Youth and Sports,
Principal investigator: Mgr. Karel Černý, Ph.D.,
Project period: 2015–2017.

Abstract
An invasive cryptogenic pathogen of uncertain systematic position Gemmamyces (Cucurbitaria) piceae, the causal agent of bud blight disease of several spruce species, is contemporary spreading within the Czech Republic and Europe. In the frame of the project its distribution, importance, systematic position, host-pathogen relationships and basics of disease epidemiology are investigated. The best period for chemical control will be also determined.

Project results

ČERNÝ, K., PEŠKOVÁ, V., SOUKUP, F., HAVRDOVÁ, L., STRNADOVÁ, V., ZAHRADNÍK, D. & HRABĚTOVÁ, M. (2016): Gemmamyces bud blight of Picea pungens: A sudden disease outbreak in Central Europe. – Plant Pathology, DOI: 10.1111/ppa.12513. WWW: http://onlinelibrary.wiley.com/doi/10.1111/ppa.12513/abstract

Continuos report of the project (Extract)
The all outcomes planned during the first stage in 2015 of the research project LD15148 were achieved. The localities with presence of the pathogen were identified within the area of the Czech Republic, the affected trees were sampled and presence of the pathogen was verified. The sampled material was microscoped partially and the data stored in the database. Some representative isolates were acquired and stored in a culture collection. rDNA was acquired from some representative isolates, the PCR reaction was optimised and primers were selected. The sequences of selected DNA sections (LSU, SSU, ITS, translation elongation factor 1α, and β-tubulin). The comparison of sequences of rDNA of acquired isolates with sequences deposited in GenBank database with use of BLAST software showed the separateness of the organism in Pleosporales (the closest sequences of ITS region showed only 92 – 94% similarity and belonged to items called “Uncultured fungus” and “Uncultured Ascomycota”). The isolate of G. piceae from CBS collection was acquired, and its rDNA extracted. The comparison of Czech material with that isolate (from Switzerland) revealed 100% similarity. The Czech material is extremely homogeneous and does not show an important genetic variability. The acquired sequences were deposited in GenBank under KT595244, KT595246 a KT595247. The 11 isolates were deposited in the collection of the institute under codes S33-39 a S48-51 and 13 herbarium items were deposited in PRC under codes PRC2977 až PRC2989. An article aimed to Plant Pathology was prepared.